Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway

Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway

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Wei-Ping Xiao,1,* Li-Li-Qiang Ding,2,* You-Jiang Min,1,3 Hua-Yuan Yang,4 Hai-Hua Yao,3 Jie Sun,1 Xuan Zhou,1 Xue-Bo Zeng,1 Wan Yu1 1Spinal Department of Orthopedics and Department of Acupuncture, Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, People’s Republic of China; 2Department of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3Department of Traditional Chinese Medicine, Shanghai Eighth People’s Hospital, Shanghai, People’s Republic of China; 4Institute of Traditional Chinese Medicine Engineering, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: You-Jiang MinShanghai Eighth People’s Hospital, Caobao Road 8, Shanghai 200235, People’s Republic of ChinaTel +86 Enhancing reproductive health among adolescent girls in India: results of an individualized RCT to study the efficacy of the go Nisha go mobile game 18221786381Email [email protected] Hua-Yuan YangShanghai University of Traditional Chinese Medicine, Cairen Road 1200, Shanghai 201203, People’s Republic of ChinaTel +86 13651968830Email [email protected]: To observe the changes of Nogo/NgR and Rho/ROCK signaling pathway-related gene and protein expression in rats with spinal cord injury (SCI) treated with electroacupuncture (EA) and to further investigate the possible mechanism of EA for treating SCI.Methods: Allen’s method was used to create the SCI rat model.Sixty-four model rats were further subdivided into four subgroups, namely, the SCI model group (SCI), EA treatment group (EA), blocking agent Y27632 treatment group (Y27632) and EA+blocking agent Y27632 treatment group (EA+Y), according to the treatment received.

The rats were subjected to EA and/or blocking agent Y27632 treatment.After 14 days, injured spinal cord tissue was extracted for analysis.The mRNA and protein expression levels were determined by real-time fluorescence quantitative PCR and Western blotting, respectively.Cell apoptosis changes in the spinal cord were evaluated by in situ hybridization.Hindlimb motor function in the rats was evaluated by Basso-Beattie-Bresnahan assessment methods.

Results: Except for RhoA protein expression, compared with the SCI model group, EA, blocking agent Y27632 and EA+blocking agent Y27632 treatment groups had significantly reduced mRNA and protein expression of Nogo-A, NgR, LINGO-1, RhoA and ROCK II in spinal cord tissues, increased mRNA and protein expression of MLCP, decreased p-MYPT1 protein expression and p-MYPT1/MYPT1 ratio, and caspase3 expression, and improved lower limb movement function after treatment for 14 days (P<0.01 or <0.05).The combination of EA and the blocking agent Y27632 was superior to EA or blocking agent Y27632treatment alone (P < 0.01 or <0.

05).Conclusion: EA may have an obvious inhibitory effect on the Nogo/NgR and Rho/ROCK signaling pathway after SCI, thereby reducing the inhibition of axonal growth, which may be a key mechanism of EA treatment for SCI.Keywords: Nogo/NgR, Rho/ROCK, MLCP, MYPT1, Decision-Making Within Forensic Psychiatric Investigations: The Use of Various Information Sources by Different Expert Groups to Reach Conclusions on Legal Insanity spinal cord injury, Y27632, electroacupuncture.